Thymine Dimer Splitting by Ionizing Radiation
نویسندگان
چکیده
منابع مشابه
Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase eta.
The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Pol eta, which inserts two adenines opposite the two thymines of a cis-syn thymine-thymine (T-T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T-T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged ...
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Photolyase uses light energy to split UV-induced cyclobutane dimers in damaged DNA, but its molecular mechanism has never been directly revealed. Here, we report the direct mapping of catalytic processes through femtosecond synchronization of the enzymatic dynamics with the repair function. We observed direct electron transfer from the excited flavin cofactor to the dimer in 170 ps and back ele...
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متن کاملMechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase eta.
DNA polymerase eta (Poleta) has the unique ability to replicate through UV-light-induced cyclobutane pyrimidine dimers. Here we use pre-steady-state kinetic analyses to examine the mechanism of nucleotide incorporation opposite a cis-syn thymine-thymine (TT) dimer and an identical nondamaged sequence by yeast Poleta. Poleta displayed "burst" kinetics for nucleotide incorporation opposite both t...
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3900 COLUMNS One of the major sources of DNA damage in all organisms is the UV component of sunlight. The predominant reaction induced by UV light on DNA is dimerization of adjacent pyrimidine bases leading to cyclobutane dimers (CPDs) and 6-4 photoproducts. The dimers formed in the most significant quantity are the cis-syn cyclobutane dimer of two thymine bases (1) and the corresponding 64 pho...
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ژورنال
عنوان ژورنال: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
سال: 1974
ISSN: 0020-7616
DOI: 10.1080/09553007414550701